GENES The gene is a nucleic acid sequence that is necessary for function of gene product. Gene may be a protein coding gene whose transcribed RNA is translated into proteins or RNA only genes. Protein coding genes may be solitary or families or tandem repeats NON –CODING SEQUENCE The non coding sequence are genes that do not encode the protein. The amount of non-coding sequence in DNA of organism varies among species. Eg-Human genome -98% non-coding genes Prokaryotes -10% non-coding genes Types of Non coding sequences The non coding sequences can be in the form of following Introns Anti leader and Anti trailer sequence Promoter sequence Enhancers Pseudogenes Gene fragments Telomeres Non-coding functional RNA Cis-Trans regulatory elements INTRONS Introns are non-coding sequence that transcribed into RNA But precursor mRNA removed by splicing during processing. The eukaryotic genome has large amount of introns...
Aminoacid reaction with NH 2 group group :
1.WITH MINERAL ACIDS:
Aminoacid with mineral acid to form aminoacid hydrochloride compounds.
2.ACYLATION:
Aminoacid with acetic anhydride and sodium hydroxide to form acylated aminoacid.
3.BENZOYLATION:
Aminoacid with benzoic acid to form hippuric acid or benzoyl glycine.
4.SANGERS REACTIIN:
Aminoacid with 1- fluro dinitro benzene in presence of sodium bicarbonate to form yellow coloured complex,which is used for aminoacid sequencing.
5.SLEGFREID CARBAMINO REACTION:
Aminoacid with carbon di oxide to form carbaminoacid which is important during oxygen and carbon di oxide transfer.
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